![]() Here is a collection of external links about Compensation.Īll of the pages linked above, also link back here for your convenience. When you are ready to proceed to a more complete overview of the new compensation work flow, please see this document.Īlso, consider the Compensation Tutorial. One other document you will likely find useful is:Īpplying/Renaming – the matrix, a small overview. The Matrix editor – used to view/adjust matrix numbers with a preview of the changes.Click the Concatenate button at the bottom of the window. The Compensation window – used to define a compensation matrix from single stain controls Expand the Advanced Options section and add a suffix indicating the antibody.The Compensation group – in the workspace and tied to compensation Preferences.Single stained controls used to compensate in FlowJo will need to be scaled correctly ahead of time.įor a more complete overview of the theory and principles of compensation, please check out this page.įor version 10, there are three main components to the user interface for Compensation: Single stain controls must be run to determine the amount of secondary fluorescence contributed to each channel. Therefore, in multi-color flow cytometry, each channel will detect the primary fluorescence, but also secondary fluorescence arising from other overlapping fluorophores. However, the fluorophores used in flow cytometry do not adhere to the exact range of emission detected by the instrument. The detectors, or channels, in the instrument are designed to detect a very specific range of emissions. Use the movie function to view parameters on a plot using a third parameter as the remote control.Compensation has undergone a major redesign for FlowJo Version 10!Ĭompensation in flow cytometry is the process of correcting for fluorescence spillover emissions.Export a movie – What better way to show data in the multimedia age than a web-ready animation of your plot?.As a note, FlowJo bins your data into 4096 channels, and then displays it in 256, so if you plot our binned data into your own histogram, there will be 4096 channels and the profile may look slightly different. ![]() Copy a histogram as text – Great for quickly pasting the binned data into your spreadsheet of choice.Copy the graph window to the clipboard with transparency.Copy/paste plots for use in other programs – Want flexibility? There are four kinds of Copy Image functions under one Edit Menu! Just a quick Hot Key away, too!.Exporting statistics and table information for use in other programs like Excel, Prism, or a database - If you make a steller table and need to show the world, export it in any format/file types or even loop it right back into the Layout Editor.Copy/paste images for use in other programs – If you only want to export some layout elements, FlowJo lets you use common file formats in simple copy-paste actions.Outlook ADVANCED FEATURES AND FUNCTIONS Microsoft Outlook ADVANCED. Send a batch operation directly to a presentation, a PDF, or even a web-ready slideshow! FlowJo Basic Tutorial FlowJo is software for the analysis of flow cytometry data. ![]() Exporting plots and batch reports – Send whole layouts to half a dozen file formats.In the Layout Editor, you can copy/paste graphs:.This isn’t so much of an “exporting” feature, but will allow you to utilize the workspace for future experiments.Exporting Workspace structure – Export your entire workspace without data to use as a template with another data set.Exporting your data in CSV – Want to crunch the raw numbers in a spreadsheet or statistical package? Try creating this text file format that is easily digested by the most finicky parsers.You can export an entire file and select the number of events you want in the new file, or you can export a gated subset and get a new file with just the events in that gated region. Just right click a file in the workspace and choose Export.Exporting new FCS files – Need to clean up data or reduce file size? Try exporting a subset of your data file into a fresh new FCS file and reimport into FlowJo.The default format for many areas’ export actions can be set in File Format Preferences. Click on the links below for more detailed information about export options for each area. The interface areas of FlowJo that have export options are listed below, along with their relevant export formats. ![]() From almost every interface of the program there is a way to get your sample data, plots, or statistics out of FlowJo for use in spreadsheets, graphics programs, or almost any secondary software package. Getting the good stuff inside FlowJo out for other uses!įlowJo makes exporting your analyses quick and easy.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |